gene fragment encoding rbd spike protein sars-cov-2, fused dc-binding peptide Search Results


rbd spike protein sars-cov-2, fused dc-binding peptide  (GenScript corporation)
90
GenScript corporation rbd spike protein sars-cov-2, fused dc-binding peptide
Rbd Spike Protein Sars Cov 2, Fused Dc Binding Peptide, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+fragment+encoding+rbd+spike+protein+sars-cov-2%2C+fused+dc-binding+peptide/cpass+sars+cov+2+neutralization+antibody+detection+kit/pm39817779-77-19-29
Average 90 stars, based on 1 article reviews
rbd spike protein sars-cov-2, fused dc-binding peptide - by Bioz Stars, 2026-10
90/100 stars
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binding buffer  (Promega)
90
Promega binding buffer
Binding Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+fragment+encoding+rbd+spike+protein+sars-cov-2%2C+fused+dc-binding+peptide/binding+buffer/pm09613613-47-14-17
Average 90 stars, based on 1 article reviews
binding buffer - by Bioz Stars, 2026-10
90/100 stars
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in vitro-translated tnt slug-flag protein  (Promega)
90
Promega in vitro-translated tnt slug-flag protein
Slug represses the endothelial phenotype and directly regulates the VE-cadherin promoter. (A) Analysis of endothelial marker expression by qRT-PCR in Slug-transduced HMEC ( n = 3). *, P < 0.05. (B) Immunoblots for endothelial and mesenchymal markers in empty vector–, NotchICD-, and Slug-expressing HMEC. (C) EMSA using in vitro–translated <t>luciferase</t> (Luc) or Slug-FLAG protein and 32 P-labeled double-stranded oligonucleotides for an E-box cis element (−97) or two putative Slug E2-box motifs (−306 and −379) in the human VE-cadherin promoter. Supershift assays with anti–FLAG-M2 or IgG control antibodies and competition assays with 50× wild-type (wt) or mutant probes are also shown. (D) Promoter activity in endothelial cells cotransfected with vector or Slug plasmids and wild-type, E2-box mutant, or E-box mutant mouse VE-cadherin promoter-luciferase constructs ( n = 4; each experiment performed in triplicate). *, P < 0.05. Error bars show SEM.
In Vitro Translated Tnt Slug Flag Protein, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+fragment+encoding+rbd+spike+protein+sars-cov-2%2C+fused+dc-binding+peptide/in+vitro+translated+tnt+slug+flag+protein/pmc02483533-182-10-7
Average 90 stars, based on 1 article reviews
in vitro-translated tnt slug-flag protein - by Bioz Stars, 2026-10
90/100 stars
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Slug represses the endothelial phenotype and directly regulates the VE-cadherin promoter. (A) Analysis of endothelial marker expression by qRT-PCR in Slug-transduced HMEC ( n = 3). *, P < 0.05. (B) Immunoblots for endothelial and mesenchymal markers in empty vector–, NotchICD-, and Slug-expressing HMEC. (C) EMSA using in vitro–translated luciferase (Luc) or Slug-FLAG protein and 32 P-labeled double-stranded oligonucleotides for an E-box cis element (−97) or two putative Slug E2-box motifs (−306 and −379) in the human VE-cadherin promoter. Supershift assays with anti–FLAG-M2 or IgG control antibodies and competition assays with 50× wild-type (wt) or mutant probes are also shown. (D) Promoter activity in endothelial cells cotransfected with vector or Slug plasmids and wild-type, E2-box mutant, or E-box mutant mouse VE-cadherin promoter-luciferase constructs ( n = 4; each experiment performed in triplicate). *, P < 0.05. Error bars show SEM.

Journal: The Journal of Cell Biology

Article Title: Slug is a direct Notch target required for initiation of cardiac cushion cellularization

doi: 10.1083/jcb.200710067

Figure Lengend Snippet: Slug represses the endothelial phenotype and directly regulates the VE-cadherin promoter. (A) Analysis of endothelial marker expression by qRT-PCR in Slug-transduced HMEC ( n = 3). *, P < 0.05. (B) Immunoblots for endothelial and mesenchymal markers in empty vector–, NotchICD-, and Slug-expressing HMEC. (C) EMSA using in vitro–translated luciferase (Luc) or Slug-FLAG protein and 32 P-labeled double-stranded oligonucleotides for an E-box cis element (−97) or two putative Slug E2-box motifs (−306 and −379) in the human VE-cadherin promoter. Supershift assays with anti–FLAG-M2 or IgG control antibodies and competition assays with 50× wild-type (wt) or mutant probes are also shown. (D) Promoter activity in endothelial cells cotransfected with vector or Slug plasmids and wild-type, E2-box mutant, or E-box mutant mouse VE-cadherin promoter-luciferase constructs ( n = 4; each experiment performed in triplicate). *, P < 0.05. Error bars show SEM.

Article Snippet: For Slug EMSA assays, in vitro–translated (TNT; Promega) Slug-FLAG or control luciferase protein was preincubated with FLAG-M2 antibody overnight at 4°C in 12 mM Hepes, pH 7.9, 4 mM Tris, pH 7.9, 133 mM KCl, 10% Glycerol, and 2 μg PolydI-dC binding buffer.

Techniques: Marker, Expressing, Quantitative RT-PCR, Western Blot, Plasmid Preparation, In Vitro, Luciferase, Labeling, Control, Mutagenesis, Activity Assay, Construct